Isolation of RNA and analysis by Northern blotting and primer extension
نویسندگان
چکیده
منابع مشابه
Analysis of RNA by primer extension.
1. Phosphorylate oligonucleotide probe: a. Oligonucleotide primer (5 – 7 pmoles or 60 ng) 1 μL b. dH2O 6.5 μL c. 10X kinase buffer 1.5 μL d. T4 PNK (~10 U) 1 μL e. [γ-P]ATP (7000 Ci/mmole) 2 μL 2. Stop the kinase reaction by adding 500 μL of TE (pH 7.6) 3. Add 25 μg of carrier RNA. 4. Phenol/Chloroform extract 2X 5. Ethanol precipitate with sodium acetate (pH 5.2) 6. Wash with 70% ethanol and r...
متن کاملa comparative move analysis of the introduction sections of ma theses by iranian and native post-graduate students
since esp received universal attention to smooth the path for academic studies and productions, a great deal of research and studies have been directed towards this area. swales’ (1990) model of ra introduction move analysis has served a pioneering role of guiding many relevant studies and has proven to be productive in terms of helpful guidelines that are the outcome of voluminous productions ...
15 صفحه اولNorthern blotting analysis.
Northern blotting analysis is a classical method for analysis of the size and steady-state level of a specific RNA in a complex sample. In short, the RNA is size-fractionated by gel electrophoresis and transferred by blotting onto a membrane to which the RNA is covalently bound. Then, the membrane is analysed by hybridization to one or more specific probes that are labelled for subsequent detec...
متن کاملlangauge needs analysis of undergraduate business management and economics students
the aim of conducting this study was to investigate the foreign language learning needs of undergraduate economics students and business management students in faculties of social sciences of alzahra and azad naragh university. in the study, which was designed on the basis of a qualitative-quantitative basis using interviews and questionnaires, 146 female undergraduate business management as we...
15 صفحه اولa contrastive analysis of concord and head parameter in english and azerbaijani
این پایان نامه به بررسی و مقایسه دو موضوع مطابقه میان فعل و فاعل (از نظر شخص و مشار) و هسته عبارت در دو زبان انگلیسی و آذربایجانی می پردازد. اول رابطه دستوری مطابقه مورد بررسی قرار می گیرد. مطابقه به این معناست که فعل مفرد به همراه فاعل مفرد و فعل جمع به همراه فاعل جمع می آید. در انگلیسی تمام افعال، بجز فعل بودن (to be) از نظر شمار با فاعلشان فقط در سوم شخص مفرد و در زمان حال مطابقت نشان میدهند...
15 صفحه اولذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Technical Tips Online
سال: 1998
ISSN: 1366-2120
DOI: 10.1016/s1366-2120(08)70087-4